Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 3. VAC alleviated the renal inflammatory response and oxidative stress in T2DM mice (A) MDA contents in diabetic kidney tissues. n=6. (B) GSH-Px activity in diabetic kidney tissues. n=6. (C) Averaged fluorescence intensity of DHE fluorescence in diabetic kidney tissues. n=6. (D) Averaged fluorescence intensity of DCFH-DA fluorescence staining of diabetic kidney tissues. n=6. (E) DHE fluorescence staining or DCFH-DA fluorescence staining of diabetic kidney tissues. Scale bar: 100 μm. (F) F4/80 staining of diabetic kidney tissues. Scale bar: 50 μm, and the average fluorescence intensity of F4/80-expressing diabetic kidney tissues is shown. (G‒M) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. n=4. *P<0.05, **P<0.01, ***P<0.001 vs Ctrl. #P<0.05, ##P<0.01, ###P<0.001 vs DN.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Activity Assay, Fluorescence, Staining, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 5. VAC alleviated the inflammatory response and oxidative stress in diabetic kidneys HK-2 cells were preincubated with 5 μM VAC for 12 h, followed by exposure to 35 mM HG for 48 h. (A–C) Relative mRNA levels of IL-1β, VCAM-1 and COX2. (D-F) DHE fluorescence staining or DCFH-DA fluorescence staining of HK-2 cells. Scale bar: 100 μm. (G–K) Relative mRNA levels of Nrf2, catalase, SOD3, SOD2 and SOD1. (L–R) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 8. VAC ameliorated fibrosis, the inflammatory response and oxidative stress in HG-induced HK-2 cells exposed to the EGFR inhibitor AG1478 or the ERK inhibitor U0126 (A–D) Relative mRNA levels of collagen-1, TGF-β1, α-SMA and E-cadherin in HK-2 cells. (E–G) Relative mRNA levels of IL-1β, VCAM-1 and COX-2 in HK-2 cells. (H) Averaged fluorescence intensity of DHE fluorescence in HK-2 cells. (I) DHE staining was performed on HG-induced HK-2 cells. Scale bar: 100 μm. (J–P) Representative blot images and quantitative analysis of phosphorylated and total NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: Psychology Research and Behavior Management

Article Title: Chronic Restraint Stress Induces Gastric Mucosal Inflammation with Enhanced Oxidative Stress in a Murine Model

doi: 10.2147/PRBM.S250945

Figure Lengend Snippet: Sequences of Primers Used for the RT-PCR Experiment

Article Snippet: Membranes were washed with TBS-T, and incubated with primary antibodies against Mn SOD (Cat no:MAB10394, Abnova, Taiwan), Cu, Zn SOD (Cat no:AF3418, R&D System, USA), Catalase (Cat no:ab52477, Abcam, UK), and GPx (Cat no:ab59524, Abcam, UK) and β-actin (Cat no: #3700, Cell Signaling Technology, USA) (all these antibodies dilution rate was 1:1000).

Techniques:

Journal: Psychology Research and Behavior Management

Article Title: Chronic Restraint Stress Induces Gastric Mucosal Inflammation with Enhanced Oxidative Stress in a Murine Model

doi: 10.2147/PRBM.S250945

Figure Lengend Snippet: Stress decreased the gastric expressions of antioxidants in mice. The mRNA and protein expression levels of antioxidant enzymes in the stomach tissue of control mice and stressed mice were analyzed by quantitative RT-PCR and Western blot, respectively ( A – E ). The values for the stress mice are presented in comparison with those for the control mice and are expressed as mean±SD ( n =15). Student’s t -test was performed to test the differences between the stress and control groups. ( A ) Quantitative analysis of Mn SOD mRNA, Cu, Zn SOD mRNA ( B ), catalase mRNA ( C ), and GPx mRNA ( D ) expression levels in stomach tissue; ( E ) protein expressions of Mn SOD, Cu, Zn SOD, catalase, and GPx in stomach tissue of control and stressed mice. ** P <0.001 compared with the control mice.

Article Snippet: Membranes were washed with TBS-T, and incubated with primary antibodies against Mn SOD (Cat no:MAB10394, Abnova, Taiwan), Cu, Zn SOD (Cat no:AF3418, R&D System, USA), Catalase (Cat no:ab52477, Abcam, UK), and GPx (Cat no:ab59524, Abcam, UK) and β-actin (Cat no: #3700, Cell Signaling Technology, USA) (all these antibodies dilution rate was 1:1000).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Comparison

Journal: Poultry Science

Article Title: Effects of melittin on production performance, antioxidant function, immune function, heat shock protein, intestinal morphology, and cecal microbiota in heat-stressed quails

doi: 10.1016/j.psj.2023.102713

Figure Lengend Snippet: Correlation analysis between health parameters and cecal microbial composition in the genus levels in quails. With SPSS Statistics 26.0, Spearman's correlation analysis was conducted, and the results were visualized with R ( https://www.xiantao.love/products ). The red and blue circles represent positive correlation and negative correlation, respectively. Abbreviations: ADFI, average daily feed intake; ADG, average daily gain; F/G, feed-to-gain ratio; GSH-Px, glutathione peroxidase; HSP, heat shock protein; MDA, malondialdehyde; SOD, superoxide dismutase. Thymus, bursa of Fabricius, and spleen refer to their organ index. * P < 0.05, ** P < 0.01.

Article Snippet: The antioxidant indexes for superoxide dismutase ( SOD ), glutathione peroxidase ( GSH-Px ), and malondialdehyde ( MDA ) and immune indexes for complement C 3 , interferon-γ ( IFN-γ ), IgG, and IgA were measured using commercial ELISA kits (Sino Best Biological Technology Co. Ltd., Shanghai, China) by following the protocol provided by the manufacturer.

Techniques: